Target- YopH (S. aureus)-------------------------------------Stephanie Bsaibes

*Target (protein/gene name): YopH (protein-tyrosine-phosphatase)
*NCBI Gene # or RefSeq#:
*Protein ID (NP or XP #) or Wolbachia#: NP_783699
*Organism: Staphylococcus aureus/ Yersinia entercolitica
Etiologic Risk Group (see link below): “usually exotoxin producing strains of Staphylococcus aureus”
*Background/Disease Information:http://www.cdc.gov/ncidod/dbmd/diseaseinfo/toxicshock_t.htm
A sudden onset of fever, chills, vomiting, diarrhea, muscle aches and rash characterize Toxic Shock Syndrome and can progress into severe hypotension. The bacterium colonizes skin and mucous membranes in humans. [for Yersinia= gastrointestinal syndromes to Bubonic Plague]
Essentiality of this protein: http://www.jbc.org/content/early/2003/07/29/jbc.M307152200.full.pdf
Complex of enzymes: no
*EC#: 3.1.3.48
Link to BRENDA EC# page: http://brenda-enzymes.org/php/result_flat.php4?ecno=3.1.3.48
Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):
http://www.sigmaaldrich.com/catalog/search?interface=All&term=protein%20tyrosine%20phosphatase&lang=en&region=US&focus=product&N=0+220003048+219853269+219853286

-- http://www.jbc.org/content/early/2003/07/29/jbc.M307152200.full.pdf (assay proof?)
-- http://www.millipore.com/coa.nsf/a73664f9f981af8c852569b9005b4eee/7c92efa9de1d3015852579e9004c7dce/$FILE/14-590_D7DN012U-G.pdf

Structure Available (PDB or Homology model)
-- PDB # or closest PDB entry if using homology model: 1K46
-- Query Coverage (if not direct match):
-- Max Ident (if not direct match):
Druggable Target (see Databases for this): Yes
Current Inhibitors: http://www.bindingdb.org/jsp/dbsearch/PrimarySearch_ki.jsp?tag=pol&submit=Search&target=tyrosine-protein%20phosphatase%20yoph&polymerid=50003487
Expression Information (has it been expressed in bacterial cells): --- http://www.ncbi.nlm.nih.gov/pubmed/19199933
-http://www.millipore.com/coa.nsf/a73664f9f981af8c852569b9005b4eee/7c92efa9de1d3015852579e9004c7dce/$FILE/14-590_D7DN012U-G.pdf

Purification Method: http://onlinelibrary.wiley.com/doi/10.1046/j.1462-5822.2000.00061.x/full#ss2
Image of protein (PyMol or etc):

Image of YopH

*Amino Acid Sequence:
>gi|28373018|ref|NP_783699.1| YopH [Yersinia enterocolitica]
MNLSLSDLHRQVSRLVQQESGDCTGKLRGNVAANKETTFQGLTIASGARESEKVFAQTVLSHVANIVLTQ
EDTAKLLQSTVKHNLNNYELRSVGNGNSVLVSLRSDQMTLQDAKVLLEAALRQESGARGHVSSHSHSVLH
APGTPVREGLRSHLDPRTPPLPPRERPHTSGHHGAGEARATAPSTVSPYGPEARAELSSRLTTLRNTLTP
ATNDPRYLQACGGEKLNRFRDIQCCRQTAVRADLNANYIQVGNTRTIACQYPLQSQLESHFRMLAENRTP
VLAVLASSSEIANQRFGMPDYFRQSGTYGSITVESKMTQQVGLGDGIMADMYTLTIREAGQKTISVPVVH
VGNWPDQTAVSSEVTKALASLVDQTAETKRNMYESKGSSAVADDSKLRPVIHCRAGVGRTAQLIGAMCMN
DNRNSQLSVEDMVSQMRVQRNGIMVQKDEQLDVLIKLAEGQGRPLLNS

*length of your protein in Amino Acids: 468
Molecular Weight of your protein in kiloDaltons using the **Expasy ProtParam** website: 50996.4
Molar Extinction coefficient of your protein at 280 nm wavelength: 19285
*CDS Gene Sequence:
ATGAACTTATCATTAAGCGATCTTCATCGTCAGGTATCTCGATTGGTGCAGCAAGAGAGCGGTGATT
GTACCGGGAAATTAAGAGGTAACGTTGCTGCCAATAAAGAAACTACCTTTCAAGGTTTGACCATAG
CCAGTGGTGCCAGAGAGTCAGAAAAAGTATTTGCTCAAACTGTACTAAGCCACGTAGCAAATATTG
TTCTAACTCAAGAAGATACCGCTAAGCTATTGCAAAGCACGGTAAAGCATAATTTGAATAATTATGAA
TTAAGAAGTGTCGGCAATGGTAATAGTGTACTTGTCAGTTTACGTAGTGACCAAATGACACTACAAG
ACGCCAAAGTGCTGTTGGAGGCTGCATTGCGACAAGAGTCGGGAGCGAGGGGGCATGTATCATC
TCATTCACATTCAGTCCTTCACGCACCGGGAACTCCGGTGCGTGAAGGACTGCGTTCACATCTAGA
CCCCAGAACACCACCGTTGCCACCGCGTGAACGACCACACACTTCTGGCCATCACGGGGCTGGC
GAAGCCAGAGCCACCGCACCAAGCACTGTTTCTCCTTATGGCCCAGAAGCGCGCGCAGAACTCA
GCAGCCGCCTCACCACATTGCGCAATACGCTGACGCCAGCAACGAATGATCCGCGTTACTTACAAG
CCTGCGGCGGTGAAAAGCTAAACCGATTTAGAGATATTCAATGCTGTCGGCAAACCGCAGTACGCG
CCGATCTTAATGCCAATTACATCCAGGTCGGTAACACTCGTACCATAGCGTGCCAGTATCCGCTACAA
TCTCAACTTGAAAGCCATTTCCGTATGCTGGCAGAAAACCGAACGCCAGTGTTGGCTGTTTTAGCGT
CCAGTTCTGAGATAGCCAATCAAAGATTCGGTATGCCAGATTATTTCCGCCAGAGTGGTACCTATGGC
AGTATCACTGTAGAGTCTAAAATGACTCAGCAAGTTGGTCTCGGTGACGGGATTATGGCAGATATGTA
TACTTTAACGATTCGTGAAGCGGGTCAAAAAACAATTTCTGTTCCTGTGGTTCATGTTGGCAATTGGCC
CGATCAGACCGCAGTCAGCTCTGAAGTTACCAAGGCACTCGCTTCACTGGTAGATCAAACAGCAGA
AACAAAACGCAATATGTATGAAAGCAAAGGAAGTTCAGCGGTAGCAGATGACTCCAAATTACGGCC
GGTAATACATTGCCGTGCGGGTGTTGGCCGTACTGCGCAACTGATTGGCGCAATGTGCATGAATGAT
AATCGTAATAGTCAGTTAAGCGTAGAAGATATGGTCAGCCAAATGCGAGTACAAAGAAATGGTATTAT
GGTACAAAAAGATGAGCAACTTGATGTTCTGATTAAGTTGGCTGAAGGACAAGGGCGACCATTATTA
AATAGCTAA

*GC% Content for gene: 46.9%
*CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol:
*GC% Content for gene (codon optimized):